![]() ![]() We created a structure-based workflow for functional annotation transfer, which we refer to as MorF (MorphologFinder). To assess MorF annotations, we compare them to those produced via EggNOG v5.0 and EggNOG-mapper, representing the state-of-the-art in homology detection. A protein structure-based workflow enriches functional annotation for Spongilla lacustris. In the absence of robust high-throughput alternatives, sequence-based homology detection remains the standard for (transitive) functional annotation. Text boxes or upload files for both Pairwise alignment and MSA. In particular, orthology has often been used to transfer functional annotation wholesale, even though it is known that divergence of function within orthologs is possible. 4peaks sequence alignment software download In this case the 100 lines of FASTA input would be compared to the The second sequence might contain one large FASTA sequence line,or example a well known bacteria chromosome. For the rest, annotations are propagated, mostly using sufficient sequence similarity as a proxy for homology. MorF annotations agree with sequence-based annotation transferĮxperimental evidence for the function of a protein is only available for a vanishingly small number of known protein sequences. S5), even considering sequence profiles (Additional file 1: Fig. MorF accurately predicts the functions of proteins with known homology in \(56\%\) Fig. Sponges are highly relevant for inferring the early history of animals, yet their proteomes remain sparsely annotated. We propose a workflow of openly available tools for the functional annotation of proteins via structural similarity ( MorF: Morpholog Finder) and use it to annotate the complete proteome of a sponge. Here we propose a workflow for protein annotation using structural similarity, exploiting the fact that similar protein structures often reflect homology and are more conserved than protein sequences. In non-model species, the sequence-based prediction of gene orthology can be used to infer protein identity however, this approach loses predictive power at longer evolutionary distances. Protein annotation is a major goal in molecular biology, yet experimentally determined knowledge is typically limited to a few model organisms. ![]()
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